Figure-Ground Segmentation Using Multiple Cues

The theme of this thesis is figure-ground segmentation. We address the problem in the context of a visual observer, e.g. a mobile robot, moving around in the world and capable of shifting its gaze to and fixating on objects in its environment. We are only considering bottom-up processes, how the system can detect and segment out objects because they stand out from their immediate background in some feature dimension. Since that implies that the distinguishing cues can not be predicted, but depend on the scene, the system must rely on multiple cues. The integrated use of multiple cues forms a major theme of the thesis. In particular, we note that an observer in our real environment has access to 3-D cues. Inspired by psychophysical findings about human vision we try to demonstrate their effectiveness in figure-ground segmentation and grouping also in machine vision

Phase-Dependent Properties of Extrasolar Planet Atmospheres

Recently the Spitzer Space Telescope observed the transiting extrasolar planets, TrES-1 and HD209458b. These observations have provided the first estimates of the day side thermal flux from two extrasolar planets orbiting Sun-like stars. In this paper, synthetic spectra from atmospheric models are compared to these observations. The day-night temperature difference is explored and phase-dependent flux densities are predicted for both planets. For HD209458b and TrES-1, models with significant day-to-night energy redistribution are required to reproduce the observations. However, the observational error bars are large and a range of models remains viable.Comment: 8 pages, 7 figures, accepted for publication in the Astrophysical Journa

Pre-existing disease: the most important factor for health related quality of life long-term after critical illness: a prospective, longitudinal, multicentre trial

Introduction The aim of the present multicenter study was to assess long term (36 months) health related quality of life in patients after critical illness, compare ICU survivors health related quality of life to that of the general population and examine the impact of pre-existing disease and factors related to ICU care on health related quality of life. Methods Prospective, longitudinal, multicentre trial in three combined medical and surgical intensive care units of one university and two general hospitals in Sweden. By mailed questionnaires, health related quality of life was assessed at 6, 12, 24 and 36 months after the stay in ICU by EQ-5D and SF-36, and information of pre-existing disease was collected at the 6 months measure. ICU related factors were obtained from the local ICU database. Comorbidity and health related quality of life (EQ-5D; SF-36) was examined in the reference group. Among the 5306 patients admitted, 1663 were considered eligible (>24 hrs in the intensive care unit, and age ≥ 18 yrs, and alive 6 months after discharge). At the 6 month measure 980 (59%) patients answered the questionnaire. Of these 739 (75%) also answered at 12 month, 595 (61%) at 24 month, and 478 (47%) answered at the 36 month measure. As reference group, a random sample (n = 6093) of people from the uptake area of the hospitals were used in which concurrent disease was assessed and adjusted for. Results Only small improvements were recorded in health related quality of life up to 36 months after ICU admission. The majority of the reduction in health related quality of life after care in the ICU was related to the health related quality of life effects of pre-existing diseases. No significant effect on the long-term health related quality of life by any of the ICU-related factors was discernible. Conclusions A large proportion of the reduction in the health related quality of life after being in the ICU is attributable to pre-existing disease. The importance of the effect of pre-existing disease is further supported by the small, long term increment in the health related quality of life after treatment in the ICU. The reliability of the conclusions is supported by the size of the study populations and the long follow-up period.

Crystal Structure of Human Cytosolic 5′-Nucleotidase II INSIGHTS INTO ALLOSTERIC REGULATION AND SUBSTRATE RECOGNITION

Cytosolic 5′-nucleotidase II catalyzes the dephosphorylation of 6-hydroxypurine nucleoside 5′-monophosphates and regulates the IMP and GMP pools within the cell. It possesses phosphotransferase activity and thereby also catalyzes the reverse reaction. Both reactions are allosterically activated by adenine-based nucleotides and 2,3-bisphosphoglycerate. We have solved structures of cytosolic 5′-nucleotidase II as native protein (2.2 A) and in complex with adenosine (1.5A) and beryllium trifluoride (2.15A). The tetrameric enzyme is structurally similar to enzymes of the haloacid dehalogenase (HAD) superfamily, including mitochondrial 5′(3′)-deoxyribonucleotidase and cytosolic 5′-nucleotidase III but possesses additional regulatory regions that contain two allosteric effector sites. At effector site 1 located near a subunit interface we modeled diadenosine tetraphosphate with one adenosine moiety in each subunit. This efficiently glues the tetramer subunits together in pairs. The model shows why diadenosine tetraphosphate but not diadenosine triphosphate activates the enzyme and supports a role for cN-II during apoptosis when the level of diadenosine tetraphosphate increases. We have also modeled 2,3-bisphosphoglycerate in effector site 1 using one phosphate site from each subunit. By comparing the structure of cytosolic 5′-nucleotidase II with that of mitochondrial 5′(3′)-deoxyribonucleotidase in complex with dGMP, we identified residues involved in substrate recognition

Constant cross section of loops in the solar corona

The corona of the Sun is dominated by emission from loop-like structures. When observed in X-ray or extreme ultraviolet emission, these million K hot coronal loops show a more or less constant cross section. In this study we show how the interplay of heating, radiative cooling, and heat conduction in an expanding magnetic structure can explain the observed constant cross section. We employ a three-dimensional magnetohydrodynamics (3D MHD) model of the corona. The heating of the coronal plasma is the result of braiding of the magnetic field lines through footpoint motions and subsequent dissipation of the induced currents. From the model we synthesize the coronal emission, which is directly comparable to observations from, e.g., the Atmospheric Imaging Assembly on the Solar Dynamics Observatory (AIA/SDO). We find that the synthesized observation of a coronal loop seen in the 3D data cube does match actually observed loops in count rate and that the cross section is roughly constant, as observed. The magnetic field in the loop is expanding and the plasma density is concentrated in this expanding loop; however, the temperature is not constant perpendicular to the plasma loop. The higher temperature in the upper outer parts of the loop is so high that this part of the loop is outside the contribution function of the respective emission line(s). In effect, the upper part of the plasma loop is not bright and thus the loop actually seen in coronal emission appears to have a constant width. From this we can conclude that the underlying field-line-braiding heating mechanism provides the proper spatial and temporal distribution of the energy input into the corona --- at least on the observable scales.Comment: 8 pages, 9 figures, accepted for publication in A&

Coronal heating through braiding of magnetic field lines

Cool stars like our Sun are surrounded by a million degree hot outer atmosphere, the corona. Since more than 60 years the physical nature of the processes heating the corona to temperatures well in excess of those on the stellar surface remain puzzling. Recent progress in observational techniques and numerical modeling now opens a new window to approach this problem. We present the first coronal emission line spectra synthesized from three-dimensional numerical models describing the evolution of the dynamics and energetics as well as of the magnetic field in the corona. In these models the corona is heated through motions on the stellar surface that lead to a braiding of magnetic field lines inducing currents which are finally dissipated. These forward models enable us to synthesize observed properties like (average) emission line Doppler shifts or emission measures in the outer atmosphere, which until now have not been understood theoretically, even though many suggestions have been made in the past. As our model passes these observational tests, we conclude that the flux braiding mechanism is a prime candidate for being the dominant heating process of the magnetically closed corona of the Sun and solar-like stars.Comment: 4 pages, 3 figures, submitted to Ap

Modification of the loops in the ligand-binding site turns avidin into a steroid-binding protein

<p>Abstract</p> <p>Background</p> <p>Engineered proteins, with non-immunoglobulin scaffolds, have become an important alternative to antibodies in many biotechnical and therapeutic applications. When compared to antibodies, tailored proteins may provide advantageous properties such as a smaller size or a more stable structure.</p> <p>Results</p> <p>Avidin is a widely used protein in biomedicine and biotechnology. To tailor the binding properties of avidin, we have designed a sequence-randomized avidin library with mutagenesis focused at the loop area of the binding site. Selection from the generated library led to the isolation of a steroid-binding avidin mutant (sbAvd-1) showing micromolar affinity towards testosterone (K_d~ 9 μM). Furthermore, a gene library based on the sbAvd-1 gene was created by randomizing the loop area between <it>β</it>-strands 3 and 4. Phage display selection from this library led to the isolation of a steroid-binding protein with significantly decreased biotin binding affinity compared to sbAvd-1. Importantly, differential scanning calorimetry and analytical gel-filtration revealed that the high stability and the tetrameric structure were preserved in these engineered avidins.</p> <p>Conclusions</p> <p>The high stability and structural properties of avidin make it an attractive molecule for the engineering of novel receptors. This methodology may allow the use of avidin as a universal scaffold in the development of novel receptors for small molecules.</p

The price of tumor control: an analysis of rare side effects of anti-CTLA-4 therapy in metastatic melanoma from the ipilimumab network

Background: Ipilimumab, a cytotoxic T-lymphocyte antigen-4 (CTLA-4) blocking antibody, has been approved for the treatment of metastatic melanoma and induces adverse events (AE) in up to 64% of patients. Treatment algorithms for the management of common ipilimumab-induced AEs have lead to a reduction of morbidity, e.g. due to bowel perforations. However, the spectrum of less common AEs is expanding as ipilimumab is increasingly applied. Stringent recognition and management of AEs will reduce drug-induced morbidity and costs, and thus, positively impact the cost-benefit ratio of the drug. To facilitate timely identification and adequate management data on rare AEs were analyzed at 19 skin cancer centers. Methods and Findings: Patient files (n = 752) were screened for rare ipilimumab-associated AEs. A total of 120 AEs, some of which were life-threatening or even fatal, were reported and summarized by organ system describing the most instructive cases in detail. Previously unreported AEs like drug rash with eosinophilia and systemic symptoms (DRESS), granulomatous inflammation of the central nervous system, and aseptic meningitis, were documented. Obstacles included patientś delay in reporting symptoms and the differentiation of steroid-induced from ipilimumab-induced AEs under steroid treatment. Importantly, response rate was high in this patient population with tumor regression in 30.9% and a tumor control rate of 61.8% in stage IV melanoma patients despite the fact that some patients received only two of four recommended ipilimumab infusions. This suggests that ipilimumab-induced antitumor responses can have an early onset and that severe autoimmune reactions may reflect overtreatment. Conclusion: The wide spectrum of ipilimumab-induced AEs demands doctor and patient awareness to reduce morbidity and treatment costs and true ipilimumab success is dictated by both objective tumor responses and controlling severe side effects

Understanding the dopant induced effects on SFX-MeOTAD for perovskite solar cells: a spectroscopic and computational investigation

SFX-MeOTAD [2,2′,7,7′-Tetrakis(N,N-di(4-methoxyphenyl)amino)-spiro-(fluorene-9,9′-xanthene)] (also known as X60) has emerged as a cost-effective alternative to the ubiquitous, but excessively-expensive, spiro-MeOTAD hole transport material (HTM) in perovskite solar cells. Using its pre-oxidised dicationic salt, SFX-(TFSI)2, a controlled concentration dependent conductivity tuning of this HTM without the requirement of air (oxygen) exposure is carried out. This study details the modifications in optical and electrical properties of this low cost HTM as a function of concentration of the dicationic salt (0-100 mol %) using UV-Vis absorption and electrical conductivity measurements. X-ray absorption and photoelectron spectroscopic investigations have been carried out to elucidate the role of the dicationic salt on the enhanced electronic properties of SFX-MeOTAD. By incorporating the dicationic SFX-(TFSI)2 it was shown that the conductivity of SFX-MeOTAD increased by 4 orders of magnitude from 2.55 × 10-8 S cm-1 to 9.4 × 10-4 S cm-1 using an optimal dopant concentration of 20.5 mol %. The degree of oxidation of SFX-MeOTAD was determined through UV-Vis absorption and consolidated by the computational calculations. XPS study reveal that doping SFX-MeOTAD with SFX(TFSI)2 results not only in oxidation of the HTM but also leads to variation in the local chemistry around carbon and nitrogen which directly influence the conductivity of the doped films. NEXAFS studies indicates that doping enhances the aromatic nature of the molecule initially but increasing the dopant concentration further affects the aromaticity and possibly the π stacking, similar to the trend seen in dopant concentration dependent conductivity of the SFX-MeOTAD films. These findings have implications on the choice of dopant concentration and counterions more generally for triarylamine based HTMs